DdeI from New England Biolabs

Product Specs
ItemDdeI
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0175S
Quantity1000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/TNAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in ArabidopsisNature CommunicationsNovember 27, 2023Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang, Qianwen SunssDRIP-seq protocol according to references34,35. In brief, 1 μg fragmented DNA (digested by DdeI (R0175S, NEB), MseI (R0525S, NEB), NlaIII (R0125S, NEB) and MboI (R0147S, NEB) endonucleases) was used for S9.6

(2) ABE8e Corrects Pax6-Aniridic Variant in Humanized Mouse ESCs and via LNPs in Ex Vivo Cortical NeuronsOphthalmology and TherapyAugust 1, 2023Bethany A. Adair, Andrea J. Korecki, Diana Djaksigulova, Pamela K. Wagner, Nina Y. Chiu, Siu Ling Lam, Tess C. Lengyell, Blair R. Leavitt, Elizabeth M. Simpsonrestriction fragment length polymorphism (RFLP) assay using AvaI (catalog R0152S, NEB) and DdeI (catalog R0175S, NEB) restriction enzymes was used. DNA was first amplified with oEMS6223 and oEMS6224 before incubation

(3) Concurrent profiling of multiscale 3D genome organization and gene expression in single mammalian cellsbioRxivJuly 27, 2023Tianming Zhou, Ruochi Zhang, Deyong Jia, Raymond T. Doty, Adam D. Munday, Daniel Gao, Li Xin, Janis L. Abkowitz, Zhijun Duan, Jian Mafollowed by adding 20 μl 10x T4 ligation buffer, 1 μl SUPERase RNase Inhibitor and 20 μl DdeI (NEB, R0175L). The sample was then incubated at 37 °C for 1 hr and centrifuged at 500x g for 3 minutes, with the

(4) Eliminating Malaria Vectors with Precision Guided Sterile MalesbioRxivJuly 26, 2023Andrea L. Smidler, Reema A. Apte, James J. Pai, Martha L. Chow, Sanle Chen, Agastya Mondal, Héctor M. Sánchez C., Igor Antoshechkin, John M. Marshall, Omar S. Akbari(5’CAGGGTGGCATTGTACG 3’) primers and the amplicon was digested with Fspl (NEB cat#R0135S) or Ddel (NEB cat#R0175S) to identify mutations by gRNAB2.1 and gRNAB2.2 respectively. To identify mutations by gRNAdsxF.1, the

(5) An upgraded method of high-throughput chromosome conformation capture (Hi-C 3.0) in cotton (Gossypium spp.)Frontiers in Plant ScienceJuly 4, 2023Jin Han, Siyuan Wang, Hongyu Wu, Ting Zhao, Xueying Guan, Lei FangP8107S); RNase A (Biosharp, BL543A); T4 DNA polymerase (NEB, M0203S); DpnII (NEB, R0543S); DdeI (NEB, R0175S); NEBuffer 3 (NEB, B7003S). 2.2.2 Kits NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L); VAHTS™

(6) A massively parallel screening platform for converting aptamers into molecular switchesNature CommunicationsApril 24, 2023Alex M. Yoshikawa, Alexandra E. Rangel, Liwei Zheng, Leighton Wan, Linus A. Hein, Amani A. Hariri, Michael Eisenstein, H. Tom Sohfrom New England Biolabs (#M0315L). Ddel restriction enzyme was ordered from New England Biolabs (#R0175L). Cy3-labeled ddUTP (5-propargylamino-ddUTP-Cy3) was ordered from Jena Bioscience (#NU-1619-CY3) and

(7) SARS-CoV-2 Restructures the Host Chromatin ArchitecturebioRxivAugust 25, 2022Ruoyu Wang et al.overnight by adding an additional 50 μl of 10X NEB buffer 2, 100U MboI (NEB, R0147M) and 100U DdeI (NEB, R0175L). After overnight incubation, the restriction enzyme was inactivated at 62 °C for 20 mins. To fill-in

(8) Translin facilitates RNA polymerase II dissociation and suppresses genome instability during RNase H2- and Dicer-deficiencyPLOS GeneticsJune 17, 2022Natalia Gomez-Escobar, Ahad A. A. Alsaiari, Hanadi A. S. Alahamadi, Othman Alzahrani, Ellen Vernon, Hussam A. E. Althagafi, Nasser S. Almobadel, David W. Pryce, Jane A. Wakeman, Ramsay J. McFarlane[24]. Genomic DNA was fragmented using DdeI (10 U/μg of DNA) for 2 hours at 37°C (New England Biolabs; R0175s). DNA samples were divided into two and one sample was treated with RNase H (New England Biolabs; M0297s)

(9) Daw1 regulates the timely onset of cilia motility during developmentDevelopmentJune 15, 2022Elizabeth A. Bearce, Zoe H. Irons, Samuel B. Craig, Colin J. Kuhns, Cynthia Sabazali, Dylan R. Farnsworth, Adam C. Miller, Daniel T. Grimeswas synthesized from plasmid DNA after restriction enzyme digestion with SacII (New England Biolabs, R0175S). Linearized plasmids were purified using a DNA Clean & Concentrator kit (Zymo Research, D4013) then

(10) A two-step adaptive walk rewires nutrient transport in a challenging edaphic environmentScience AdvancesMay 20, 2022Emmanuel Tergemina et al.10 707 874) and NRAMP1 (Chr1: 30 374 389) by using the restriction enzymes Dde I (NEB, catalog no. R0175S) and Sac I–HF (NEB, catalog no. R3156L), respectively. We used dCAPS finder 2.0 (http://helix.wustl.edu/dcaps/dcaps.html)

(11) NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotencyNature cell biologyMay 1, 2022Choi KJ, Quan MD, Qi C, Lee JH, Tsoi PS, Zahabiyon M, Bajic A, Hu L, Prasad BVV, Liao SJ, et al.overnight by adding 50 μl of 10X NEB buffer 2, 250 U of MboI (NEB, R0147M) and 250 U of DdeI (NEB, R0175L). After overnight incubation, the restriction enzymes were inactivated at 62 °C for 20 min. To fill

(12) Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombinationMolecular CellOctober 1, 2021Jasvinder S. Ahuja, Catherine S. Harvey, David L. Wheeler, Michael Lichten,New England Biolabs,R0533L HaeIII,New England Biolabs,R0108L NdeI,New England Biolabs,R0111S DdeI,New England Biolabs,R0175S PstI,New England Biolabs,R0140S PvuII,New England Biolabs,R0151S StuI,New England Biolabs,R0187S BamHI,New England Biolabs

(13) Hi‐C 3.0: Improved Protocol for Genome‐Wide Chromosome Conformation CaptureCurrent ProtocolsJuly 21, 2021Denis L. Lafontaine, Liyan Yang, Job Dekker, Johan H. Gibcus(Fisher, cat. no. BP13111)10% Triton X‐100 (Sigma, cat. no. 93443)DdeI (New England Biolabs, cat. no. R0175L)DpnII (New England Biolabs, cat. no. R0543M)10 mM dCTP (Invitrogen, cat. no. 56173)10 mM dGTP (Invitrogen,

(14) RAC1B modulates intestinal tumourigenesis via modulation of WNT and EGFR signalling pathwaysNature communicationsApril 20, 2021Gudiño V, Pohl SÖ, Billard CV, Cammareri P, Bolado A, Aitken S, Stevenson D, Hall AE, Agostino M, Cassidy J, et al.normal murine small intestine tissue. Primers containing the sequences of Eco-RI-HF and SacII-HF (NEB, R0175S) restriction enzymes were used for Rac1b and Rac1 amplification. Primer sequences were as follows: Rac1/Rac1b

(15) Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force MicroscopyBIO-PROTOCOLMarch 5, 2021Yaqing Wang, Zhiqiang Sun, Piero R Bianco, Yuri L Lyubchenko(Qiagen, catalog number: 28104) 11. Restriction endonuclease: DdeI (New England Biolabs, catalog number: R0175S) 12. Restriction endonuclease: BspQI (New England Biolabs, catalog number: R0712S) 13. CutSmart® Buffer

(16) Identification of SRSF10 as a regulator of SMN2 ISS-N1Human mutationMarch 1, 2021Frederiksen SB, Holm LL, Larsen MR, Doktor TK, Andersen HS, Hastings ML, Hua Y, Krainer AR, Andresen BS.5´-ATATGTCCAGATTCTCTTGATG-3´ and SMN.endo.ex8as 5´-ACCATTTGAAACATTTTAAGAC-3´. PCR product was digested with Ddel (R0175S, New England Biolabs) to distinguish their origin (SMN1 or SMN2). All PCR products were either quantified

(17) Cadherin-Mediated Cell Coupling Coordinates Chemokine Sensing across Collectively Migrating CellsCurrent BiologyAugust 1, 2019Tugba Colak-Champollion, Ling Lan, Alisha R. Jadhav, Naoya Yamaguchi, Gayatri Venkiteswaran, Heta Patel, Michael Cammer, Martin Meier-Schellersheim, Holger KnautHR),National Diagnostics,Cat No EC-205 (100g) BsrI,New England Biolabs,R0527S DdeI,New England Biolabs,R0175S Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, ,, ,, ,, ,, Deposited

(18) Prevalence of the Beta Case in Variants among Tharparkar, Rathi, Sahiwal, Kankrej and Cross Breed and its Influence under Selective PressureInternational Journal of Current Microbiology and Applied SciencesMarch 20, 2019Mrinalini Saran, Ankita Gurao, Rajeev Kumar Joshi, S.K. Kashyap2.5μl of 10× NEB cutsmart buffer, 5U of TaqI enzyme for CASB67/122 primer product and 5U of DdeI (NEB # R0175S) enzyme for Csn4F/Dde4R primer product and incubated for 20-25 minutes. The restricted products were

(19) Characterization of Bubaline Leptin Gene Polymorphism By Using PCR-RFLPAlınteri Zirai Bilimler DergisiJune 30, 2018SELÇUK KAPLANR AGGCTGCACAGCTTCCTC PCR-RFLP analysis For Lep/DdeI genotyping, 20 μl of PCR product were digested with 0,5 μl (5 U) of DdeI (R0175S-New England Biolabs) restriction enzymes at 37°C for 6 hours. DdeI enzyme cuts the T allele, not the

(20) Whole-exome sequencing uncovers oxidoreductases DHTKD1 and OGDHL as linkers between mitochondrial dysfunction and eosinophilic esophagitisJCI insightApril 19, 2018Sherrill JD, Kc K, Wang X, Wen T, Chamberlin A, Stucke EM, Collins MH, Abonia JP, Peng Y, Wu Q, et al.by PCR and left untreated or treated with the restriction enzyme DdeI (New England Biolabs, catalog R0175S) for 1 hour at 37°C. Fragmentation patterns were analyzed by electrophoresis using a 1% agarose gel. DHTKD1

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DdeI

DdeI from New England Biolabs

Sequence

New England Biolabs

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DdeI from New England Biolabs

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